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Brief Description:
Gene Gun Mediated Gene Transformation| Particle Delivery System offered by Scientz, Your alternative choice besides Bio-Rad, comparable capability, much lower cost.
The gene gun was created as a new way of gene transformation using pressurized helium to accelerate DNA- or RNA-coated gold particles and delivery them into virtually any target cell or tissue. Gene gun transformation is much easier, faster, safer and more efficient.
Gene guns are so far mostly applied for plant cells. However, there is much potential use in animals and humans as well.
Features:
- Particle delivery is a convenient method , much easier and faster
- Almost no requirement to target cells
- Much more efficient and capable, and less DNA cosumed
- No carrier DNA required; no extraneous genes or proteins delivered
- Both Transient and stable gene expression are possible
- Maximal control the procedures of experiment and High reproducibility
- Removable sample room, convenient for sterilization and overpressure gas auto-release.
- Consumptive Material use-cost is much lower
Specifications:
| Experimental conditions: | in vitro, in vivo, ex vivo | | Target area: | 40cm2 | | Pressure Range: | 3-12Mpa | | Target types: | Animals: cell explant, and organ culture | | | Plants: small whole plant, plant cell culture, explant, yeast, bacteria, chloroplast, mitochondria and other microbes. | | Power voltage: | 220/110V | | Gas: | Helium or Nitrogen (He preferred) | | Metal Particle Size: | Ø0.6-1.6um (Ø1um normally) | More info about Scientz Gene Gun Scientz is one of the only two manufacturers in the world for Gene Gun System, next to Bio-Rad. We supply desk-top and hand-held gene gun system to our domestic customers over ten years. Applying Scientz gene gun system, many top laboratories made gene transformation research with impressive results. And many important thesis have been published on the professional magazines home and abroad. Scientz owned the patent of this gene gun system, patent no. 2L99124246.7
Here bellowed is a experiment to compare Scientz Gene Gun with Rio-rad which help you to get a general ideal about our product.
Experimenter: Biotechnology Research Institute from Zhejiang University
Material: Rice callus, PCAMBIA2301(11621bp, CaMV35S and GUS)
Equipment: Scientz GJ-1000, Bio-Rad PDS-1000/He, Leitz company Kryostar1720
Procedure:
I.Hyperosmotic rice callus 4 hours before bombardment
II.Scientz GJ-1000 bombardment setting: Helium pressure 7.0Mpa, Target distance 60mm, Vacuum -0.095Mpa
III.Bio-Rad PDS-1000/He bombardment setting: Pressure 1100psi, Target distance 90mm, Vacuum -26inHg Repeat this experiment 3 times and 11 Petri dishes be bombarded (about 500 rice callus)
After 24hrs recovery, examining GUS activity, furthermore freezing the rice callus with blue stain and cutting with Leitz company Kryostar1720
Results:
I. GUS examination shows that:
Transient expression rate by Scientz GJ-1000 : 45%
Transient expression rate by Bio-Rad PDS-1000/He: 47%
See Figure 1
| Figure 1 |  |  | | GJ-1000 | PSD-1000/He |
II. section of frozen tissue shows that:
The Particle be delivered into similar cellular layer after bombardment. See Figure 2
| Figure 2 |  | | GJ-1000 | PSD-1000/He |
Conclusion:
Scientz GJ-1000 gene gun has comparable capability to PSD-1000/He by Bio-Rad
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